Experiences with tonsil biopsy as an antemortem diagnostic test for detecting porcine reproductive and respiratory syndrome virus infection in breeding swine
نویسندگان
چکیده
Methods: Tonsil biopsies were collected from 42 sows in a 1500-sow breeding herd known to be chronically infected with PRRSV. Animals were euthanized after the biopsy, and tissues were collected using routine necropsy procedures. Two sows that originated from a PRRSV-negative farm were inoculated by the intranasal (IN) route with a total dose of 102.4 TCID50 of PRRSV, and a third sow was inoculated IN with virus-free cell culture medium. Serum was collected at 2to 3-day intervals throughout the study, and tonsil biopsies were taken from each animal on day 14 post infection. The animals were euthanized and necropsied. All samples were examined histologically and tested for PRRSV by Taqman polymerase chain reaction and virus isolation.
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